Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus

Hemicellulose is a major component of plant cell walls and xylan the most predominant polysaccharide. Xylan degrading enzymes integrate xylanolytic system. Xylanolytic were produced by fermentation Aspergillus niger Thermomyces lanuginosus grown on grass considered unsuitable for farming called Spartina argentinensis. Significant parameters: type used (SmF or SSF), leaves S. argentinensis (green senescent) conidia final concentration xylanase production screened optimized. The main results showed that highest levels enzyme obtained 1 × 105 conidia/mL A. in SmF at 96 h, 30ºC, with mixture 20.20% senescent 79.80% green leaves. specific activity was 62 U/mg, higher than (23 U/mg) previous work (Taddia et al., 2019), over glucanase maximized five times. optimized enzymatic extract characterized LC-MS HPLC carbohydrates. Six identified as constituents complex seven Moreover, stable 30 days 20 °C. Thus, can be within framework circular economy, rendering synergistic combination industrial applications.